Investigation into the Use of Short-Column Reversed-Phase LC in the Determination of log P Values 2.

Aim To illustrate how gas chromatography (GC) may be used for the identification and quantification of individual constituents of a complex natural product. Introduction Gas chromatography (GC) separates mixtures into their individual components and is particularly useful for volatile and semi-volatile compounds. GC is used in the analysis of a wide variety of samples, including paints, polymer additives, solvents, hydrocarbons, pesticides, and drugs. Samples are introduced to the stationary phase of the capillary column as …

…primary importance for drugs that exert their therapeutic effect on the brain. This is because to pass through the blood brain barrier, the drug must be highly lipophilic. E.G. barbiturates such as phenytoin for the treatment of epilepsy, which has it effect on the GABAa pathway in the mesolimbic pathway. References ▪Pharmaceutical analysis, Watson, Churchill Livingston 1999 ▪www.shu.ac.uk/schools/sci/chem/tutorials/chrom/gaschrm.htm ▪www.pharm.uky.edu